Journal of Biochemicals and Phytomedicine 2958-8561 5 1 2026 05 05 Biochemical and Antimicrobial Studies of Metallic Nanoparticles Synthesized from Justicia carnea Extract: Molecular Docking of Its Phytochemicals for Anti-Anaemic Potential 1 15 10.34172/jbp.2026.1 EN Adebayo Seun Gbadamosi https://orcid.org/0000-0002-5075-6530 Oloketuyi Kikelomo Boluwatife https://orcid.org/0009-0004-3774-6145 Margaret Oladunni Oseni https://orcid.org/0009-0000-1276-396X Olatunde Abass Oseni https://orcid.org/0000-0002-4284-1161 Journal Article 10.34172/jbp.2026.1 2025 05 29 2025 09 10 Introduction: Anaemia affects over 1.6 billion people worldwide. This study explored Justicia carnea and its green-synthesized metallic nanoparticles as potential anti-anaemic agents, combining biochemical evaluation and molecular docking of key phytochemicals Methods: Phytochemical composition, antioxidant capacity, antimicrobial activity, and green synthesis of three metallic NPs were analyzed using standard techniques. Eight phytochemicals were docked against anaemia-related targets (EPOR, MIF, ERα, transferrin, DHFR) using SwissDock. Binding interactions were profiled with PLIP, and drug-likeness predicted with SwissADME. Results: Screening revealed bioactive constituents including steroids, alkaloids, flavonoids, phenols, and terpenoids, indicating broad medicinal potential. The extract showed strong antioxidant activity and free-radical scavenging, potentially mitigating oxidative stress associated with anaemia. Significant antifungal activity was observed in the extract, whereas the NPs exhibited notable antibacterial effects. FTIR confirmed phenolics, carboxylic acids, and amines. Docking revealed ursolic acid binding EPOR (ΔG = −7.82 kcal/mol), rosmarinic acid interacting with ERα, β-amyrin and ursolic acid targeting MIF, and rutin binding transferrin (ΔG = −8.69 kcal/mol), supporting iron transport. Kaempferol and rosmarinic acid displayed favorable ADME profiles, suggesting drug-like potential. Conclusion: J. carnea and its biosynthesized nanoparticles demonstrate significant antioxidant, antimicrobial, and anti-anaemic activities, highlighting their potential as safe, cost-effective, and multifunctional therapeutic agents against anaemia.